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| Sciani,Juliana Mozer; Antoniazzi,Marta Maria; Neves,Adriana da Costa; Pimenta,Daniel Carvalho. |
| Background Echinometra lucunter is a common American sea urchin responsible for the majority of the marine accidents in Brazil. Although not lethal, these accidents are reported to be extremely painful. Recently, our group described the presence of toxins in its spines that contribute to the pathological reactions. Additionally, we have observed that the E. lucunter spines can regenerate when broken. In the present work we evaluated the enzymatic activities of sea urchin spine extracts in order to identify an enzyme that could contribute not only to the toxicity, but also participate in the spine growth and regeneration. Results The spine aqueous extract was tested for peptidase activity, with synthetic substrates, in the presence and absence of inhibitors... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Echinometra lucunter; Spines; Cathepsin; Proteolysis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100318 |
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| Sciani,Juliana Mozer; Zychar,Bianca; Gonçalves,Luis Roberto; Giorgi,Renata; Nogueira,Thiago; Pimenta,Daniel Carvalho. |
| Abstract Background Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine puncture in the skin. In order to characterize such toxic effects, we isolated one molecule that caused inflammatory and nociceptive effects. Methods E. lucunter specimens were collected without gender distinction. Spines were removed and molecules were extracted, fractionated by RP-HPLC and assayed for inflammatory and nociceptive activity, in a biological-driven fractionation way, until the obtainment of one active molecule and its subsequent... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxins; Sea urchin; Echinometra lucunter; Spines; Inflammation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100318 |
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